Total Cholesterol Microplate Assay Kit

Background/Introduction: Chitinases (EC 3.2.1.14) are hydrolytic enzymes that break down glycosidic bonds in chitin. Chitinase catalyzes the hydrolytic cleavage of the beta-1-->4-glycoside bond present in biopolymers of N-acetylglucosamine, primarily in chitin. Chitinases are widely distributed in living organisms and are found in fungi, bacteria, parasites, plants, and animals. They are classified in families based on amino acid sequence similarities. As chitin is a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods), chitinases are generally found in organisms that either need to reshape their own chitin or dissolve and digest the chitin of fungi or animals. Chitinases perform different functions in different organisms. In bacteria, they are mainly involved in nutritional processes. In yeast and various fungi, these enzymes participate in morphogenesis. In animals and plants, chitinases primarily play a role in the defense of the organism against pathogen attack. The assay is initiated with the enzymatic hydrolysis of the chitin by chitinases. The enzyme catalysed reaction products N-acetylglucosamine react with PDAB, and can be measured at a colorimetric readout at 585 nm.

Small volumes of TC assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS8305378 is an assay kit targeting TC. Also known as Total Cholesterol Microplate Assay Kit. Reactive in General.