Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)
Référence E-BC-K020-M-96T
Conditionnement : 96T
Marque : Elabscience
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Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method) (E-BC-K020-M)
For research use only.
| Detection Principle | The assay is based on the inhibition of WST-1 reduction by SOD. Superoxide anions generated in the reaction system reduce WST-1 to a water-soluble formazan dye. SOD activity negatively correlates with the formazan dye formation, which is quantified colorimetrically at 450 nm. |
| Detection Principle Image | |
| Synonyms | T-SOD |
| Sample Type | Serum,Plasma,Hydrothorax,ascites,Urine,Cell,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm) |
| Research Area | Oxidative Stress , Plant Physiology Series , Ferroptosis |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.2 U/mL |
| Detection Range | 0.2-14.4 U/mL |
| Precision | inter-assay CV: 3.7% | intra-assay CV: 2.9% |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 3-5 |
| Rat serum | 20-30 |
| Urine | 1 |
| Human hydrothorax | 2 |
| Cell culture supernatant | 2-3 |
| 10% Rat liver tissue homogenization | 340-370 |
| 10% Rat heart tissue homogenization | 80-100 |
| 10% Rat kidney tissue homogenization | 100-120 |
| 10% Rat brain tissue homogenization | 50-100 |
| HepG2 cells homogenization (3 mgprot/mL) | 30-40 |
| 10% Plant tissue homogenization | 5-10 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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