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> Troponin I, Cardiac, aa87-91 (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3) (HRP)

Troponin I, Cardiac, aa87-91 (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3) (HRP)

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Référence T8665-19D-HRP-100ul

Conditionnement : 100ul

Marque : US Biological

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T8665-19D-HRP Troponin I, Cardiac, aa87-91 (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3) (HRP)

Clone Type
Monoclonal
Host
mouse
Source
human
Isotype
IgG1
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Bo Ca Fe Gt Hu Po Rb
Shipping Temp
Blue Ice
Storage Temp
-20°C

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character.

The tissue specific subtypes are:
Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042)
Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043)
Cardiac troponin I, TNNI3 (19q13.4, 191044)

Applications:
Suitable for use in ELISA, Western Blot and Immunoaffinity Purification. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
SP2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 1.BB.146|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).|Purity: >90% Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.|Immunogen: Free human cardiac troponin I.|Specificity: Recognizes free human cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation, oxidation and complex formation. Recognizes an epitope located between amino acid residues 87 and 91 of cTnI. No crossreactivity with skeletal muscle troponin I. Species Crossreactivity: bovine, porcine, goat, canine, rabbit and feline. Not reactive with cTnI from rat, mouse or fish.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Free human cardiac troponin I.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).
Purity
>90% Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.
Specificity
Recognizes free human cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation, oxidation and complex formation. Recognizes an epitope located between amino acid residues 87 and 91 of cTnI. No crossreactivity with skeletal muscle troponin I. Species Crossreactivity: bovine, porcine, goat, canine, rabbit and feline. Not reactive with cTnI from rat, mouse or fish.