Principle of the Assay: The test kit is a solid phase enzyme immunometric assay (ELISA) in the microplate format, designed for the quantitative measurement of canine TSH. The microplate is coated with an antibody specific for canine TSH. Calibrators and samples are pipetted into the antibody coated microplate. Afterwards, a horseradish peroxidase-labeled antibody is added. During a two hour incubation sandwich complexes consisting of the two antibodies and the canine TSH is formed. Non-reactive components are removed by a washing step. A chromogenic substrate, TMB (3,3',5,5'-Tetra-Methyl-Benzidine), is added to all wells. During a 30 minutes incubation, the substrate is converted to a colored end product (blue) by the bound enzyme. Enzyme reaction is stopped by dispensing hydrochloric acid as stop solution (change from blue to yellow). The color intensity is direct proportional to the concentration of canine TSH present in the sample. The optical density of the color solution is measured with a microplate reader at 450 nm.

Intended Uses: TSH canine ELISA is an enzyme immunoassay for the quantitative measurement of canine TSH (thyrotropin) in serum and EDTA-plasma. For manual processing! The usage of laboratory automats is the user's sole responsibility. The kit is intended for single use only.

Small volumes of TSH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS495053 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TSH, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TSH. The ELISA analytical biochemical technique of the MBS495053 kit is based on TSH antibody-TSH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TSH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TSH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).