Tumor Necrosis Factor alpha, TNF-alpha BioAssay™ ELISA Kit (Chicken)

For the quantitative determination of chicken tumor necrosis factor alpha in serum, plasma, and tissue homogenates.

Sensitivity:
0.27pg/ml

Detection Range:
0.27-200pg/ml

Specificity:
This assay has high sensitivity and excellent specificity for detection of chicken TNF-alpha. No significant cross-reactivity or interference between chicken TNF-alpha and analogues was observed.

Assay Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TNF-alpha. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody specific to TNF-alpha. The competitive inhibition reaction is launched between with pre-coated TNF-alpha and TNF-alpha in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of TNF-alpha in the sample. The color development is stopped and the intensity of the color is measured.

Kit Components:
Microtiter Strips, 1x96 wells
Standard (Freeze dried), 2xvial
HRP-conjugate (100X), 1x60ul
HRP-conjugate Diluent, 1x20ml
Sample Diluent, 2x20ml
Wash Buffer (25X), 1x20ml
TMB Substrate, 1x10ml
Stop Solution, 1x10ml

Storage and Stability:
Store all kit components at 4°C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.