TUNEL In Situ Apoptosis Kit (HRP-DAB Method)
Référence E-CK-A331-100
Conditionnement : 100Assays
Marque : Elabscience
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Téléphone : +1 850 650 7790
For research use only.
| Detection Principle | When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis. |
| Detection Method | Colorimetric method |
| Sample Type | Paraffin section;frozen section;cell slide |
| Assay Time | 3 hours |
| Detection Instrument | Light microscope |
| Other Reagents Required | 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),Xylene，Ethanol，PBS(E-BC-R187),ddH2O,Hematoxylin(E-IR-R120)，Neutral gum(E-IR-R118) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Expiration date | 12 months |
| Shipping | Ice bag |
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