TYR siRNA (Human)
Référence MBS828810-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
TYR siRNA – Tyrosinase (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828810MBS828810 is an siRNA targeting TYR. Also known as TYR siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit TYR expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
TYR, siRNA
Full Product Name
TYR siRNA (Human)
Product Gene Name
TYR sirna
Product Synonym Names
Tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
TYR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
42,914 Da
OMIM
103470
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TYR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for TYR sirna
RNA Interference (RNAi)
Other Notes
Small volumes of TYR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for TYR. It may not necessarily be applicable to this product.
NCBI Official Full Name
tyrosinase
NCBI Official Synonym Full Names
tyrosinase
NCBI Official Symbol
TYR
NCBI Official Synonym Symbols
ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
UniProt Protein Name
Tyrosinase
UniProt Synonym Protein Names
LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB
Protein Family
Tyramine beta-hydroxylase
UniProt Gene Name
TYR
UniProt Entry Name
TYRO_HUMAN
NCBI Protein Information
tyrosinase
NCBI Summary for TYR
The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008]
Show more UniProt Comments for TYR
TYR: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. Increased expression after UVB irradiation. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; Membrane protein, integral; Amino Acid Metabolism - tyrosine; Cofactor and Vitamin Metabolism - riboflavin; EC 1.14.18.1
Chromosomal Location of Human Ortholog: 11q14.3
Cellular Component: perinuclear region of cytoplasm; lysosome; melanosome membrane; cytoplasm; integral to membrane; melanosome; Golgi-associated vesicle
Molecular Function: protein binding; copper ion binding; protein homodimerization activity; protein heterodimerization activity; monophenol monooxygenase activity
Biological Process: cell proliferation; thymus development; visual perception; melanin biosynthetic process from tyrosine; eye pigment biosynthetic process
Disease: Albinism, Ocular, With Sensorineural Deafness; Albinism, Oculocutaneous, Type Ib; Albinism, Oculocutaneous, Type Ia; Skin/hair/eye Pigmentation, Variation In, 3
Show more Protein type: Oxidoreductase; Membrane protein, integral; Amino Acid Metabolism - tyrosine; Cofactor and Vitamin Metabolism - riboflavin; EC 1.14.18.1
Chromosomal Location of Human Ortholog: 11q14.3
Cellular Component: perinuclear region of cytoplasm; lysosome; melanosome membrane; cytoplasm; integral to membrane; melanosome; Golgi-associated vesicle
Molecular Function: protein binding; copper ion binding; protein homodimerization activity; protein heterodimerization activity; monophenol monooxygenase activity
Biological Process: cell proliferation; thymus development; visual perception; melanin biosynthetic process from tyrosine; eye pigment biosynthetic process
Disease: Albinism, Ocular, With Sensorineural Deafness; Albinism, Oculocutaneous, Type Ib; Albinism, Oculocutaneous, Type Ia; Skin/hair/eye Pigmentation, Variation In, 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with TYR sirna
| Reference Product | PubMed Publications |
|---|---|
| TAT sirna | >98 publications with TYR and TAT |
| PAH sirna | >55 publications with TYR and PAH |
| MLANA sirna | >16 publications with TYR and MLANA |
| PMEL sirna | >12 publications with TYR and PMEL |
| TPO sirna | >10 publications with TYR and TPO |
| GOT1 sirna | >5 publications with TYR and GOT1 |
| DDC sirna | >5 publications with TYR and DDC |
Pathways associated with TYR sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| (S)-reticuline Biosynthesis Pathway antibodies | (S)-reticuline Biosynthesis Pathway Diagram |
| (S)-reticuline Biosynthesis II Pathway antibodies | (S)-reticuline Biosynthesis II Pathway Diagram |
| Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies | Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram |
| Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies | Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram |
| Dopamine Metabolism Pathway antibodies | Dopamine Metabolism Pathway Diagram |
| L-dopachrome Biosynthesis Pathway antibodies | L-dopachrome Biosynthesis Pathway Diagram |
| L-dopachrome Biosynthesis Pathway antibodies | L-dopachrome Biosynthesis Pathway Diagram |
| Melanin Biosynthesis Pathway antibodies | Melanin Biosynthesis Pathway Diagram |
| Melanogenesis Pathway antibodies | Melanogenesis Pathway Diagram |
| Melanogenesis Pathway antibodies | Melanogenesis Pathway Diagram |
Diseases associated with TYR sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >820 publications with TYR and Nervous System Diseases |
| Cardiovascular Diseases Antibodies | >430 publications with TYR and Cardiovascular Diseases |
| Pain Antibodies | >410 publications with TYR and Pain |
| Inflammation Antibodies | >388 publications with TYR and Inflammation |
| Melanoma Antibodies | >350 publications with TYR and Melanoma |
| Neoplasms, Experimental Antibodies | >283 publications with TYR and Neoplasms, Experimental |
| Liver Diseases Antibodies | >222 publications with TYR and Liver Diseases |
| Necrosis Antibodies | >217 publications with TYR and Necrosis |
| Hypertension Antibodies | >211 publications with TYR and Hypertension |
| Kidney Diseases Antibodies | >180 publications with TYR and Kidney Diseases |
Organs/Tissues associated with TYR sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Skin Antibodies | >549 publications with TYR and Skin |
| Eye Antibodies | >289 publications with TYR and Eye |
| Thyroid Antibodies | >181 publications with TYR and Thyroid |
| Connective Tissue Antibodies | >140 publications with TYR and Connective Tissue |
| Mammary Gland Antibodies | >41 publications with TYR and Mammary Gland |
| Ear Antibodies | >29 publications with TYR and Ear |