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Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

U0126 [109511-58-2]

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Référence NB-64-10891-25mg

Conditionnement : 25mg

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U0126

(Synonyms: U-0126, U 0126) Copy Product Info

Synonyms: U-0126, U 0126

Catalog No. T21332 Copy Product Info

Purity: 99.61%

U0126, an effective and selective non-competitive inhibitor of MAP kinase, inhibits MEK-1 and MEK-2 with IC50 values of 0.07 and 0.06 μM respectively. U0126 inhibits autophagy and mitophagy.

Cas No. 109511-58-2

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.61%

Appearance:Solid

Color:White to Brown

Product Introduction

Bioactivity

Description	U0126, an effective and selective non-competitive inhibitor of MAP kinase, inhibits MEK-1 and MEK-2 with IC50 values of 0.07 and 0.06 μM respectively. U0126 inhibits autophagy and mitophagy.
Targets & IC50	MEK1:70 nM (IC50), MEK2:60 nM (IC50)
In vitro	Rat hepatocarcinoma cells (FAO) stimulated by fetal calf serum (FCS) exhibits a significant proportion in S phase (32.62%) whereas U0126 strongly decreases the proportion of cells in S phase (9.92%) and increases the proportion of cells in G0-G1 phase and to a lesser extent in G2/M[1]. U0126 efficiently reduces progeny virus titers of all tested strains in A549 cells. While nM concentrations of U0126 are efficient to reduce H1N1v and H5N1 (MB1), μM concentrations of U0126 are required to reduce the virus titer of H5N1 (GSB) and H7N7. The EC50 values for U0126-EtOH against H1N1v are 1.2 μM in A549 cells and 74.7 μM in MDCKII cells[2].
In vivo	In mice, U0126-EtOH (U0126; i.p., 10.5 mg/kg) inhibits tumor growth with a 60-70% reduction 9 days after injection and thereafter[1]. After treatment with U0126-EtOH(U0126; i.p., 30 mg/kg), the vasoconstriction to S6c is markedly reduced in rats[3].
Synonyms	U-0126, U 0126

Chemical Properties

Molecular Weight	380.49
Formula	C₁₈H₁₆N₆S₂
Cas No.	109511-58-2
Smiles	N#CC(=C(SC=1C=CC=CC1N)N)C(C#N)=C(SC=2C=CC=CC2N)N
Relative Density.	1.44 g/cm3

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 83.33 mg/mL (219.01 mM)

H2O: Soluble

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6282 mL	13.1409 mL	26.2819 mL	131.4095 mL
5 mM	0.5256 mL	2.6282 mL	5.2564 mL	26.2819 mL
10 mM	0.2628 mL	1.3141 mL	2.6282 mL	13.1409 mL
20 mM	0.1314 mL	0.6570 mL	1.3141 mL	6.5705 mL
50 mM	0.0526 mL	0.2628 mL	0.5256 mL	2.6282 mL
100 mM	0.0263 mL	0.1314 mL	0.2628 mL	1.3141 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

U0126MitophagyMitogen-activated protein kinase kinaseMitochondrial AutophagyMEK2MEK1MEKMAPKKMAP2KInhibitorinhibitInfluenza VirusAutophagy

Related Tags: U0126 chemical structure | U0126 in vivo | U0126 in vitro | U0126 formula | U0126 molecular weight