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> Vaccinia Virus (Lister Strain) Rabbit Polyclonal Antibody

Vaccinia Virus (Lister Strain) Rabbit Polyclonal Antibody

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Référence BP1076F

Conditionnement : 1ml

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Vaccinia Virus (Lister Strain) Rabbit Polyclonal Antibody

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SKU
BP1076F
Vaccinia Virus (Lister Strain) rabbit polyclonal antibody, FITC
Size:
1 ml
Conjugation:
FITC Unconjugated Biotin
 
Specifications
Product Data
Application ELISA, IF, IHC
Recommended Dilution ELISA
Immunofluorescence: Direct FA staining of target antigens in a permissive tissue culture system.
A starting range of 1/10-1/50 is suggested.
Immunohistochemistry (Proteinase K digestion is recommended with formalin-fixed paraffin-embedded sections).
Reactivity Vaccinia virus
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Lister Strain (mixture of virions and infected cell polypeptides).
Specificity Recognizes purified virions.
Does not cross-react with Parainfluenza (1-3), RSV, adenovirus, Influenza A & B or HSV-1.
Does not react with uninfected cells.
Reactive with Lister, Wyeth, New York City and MVA strains of Vaccinia.
Buffer 0.01M PBS, pH 7.2
Label: FITC
State: Liquid purified IgG fraction
Stabilizer: 10 mg/ml BSA
Preservative: 0.09% Sodium Azide
Label: Covalently coupled with high purity Isomer I of Fluorescein Isothiocyanate Care is taken to ensure complete removal of any free fluorescein from the final product
Concentration lot specific
Purification Affinity Chromatography
Conjugation FITC
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Background Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As Vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During Vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize Vaccinia virus infection and enable identification other viral and cellular proteins which participate in the Vaccinia virus entry process.
Reference Data
Product Manuals
FAQs
  • Antibody and Western Standard
SDS

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