Vascular Endothelial Growth Factor A (VEGFA) BioAssay™ ELISA Kit (Multi Species)
Référence 517753-96T
Conditionnement : 96Tests
Marque : US Biological
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517753Vascular Endothelial Growth Factor A (VEGFA) BioAssay™ ELISA Kit (Multi Species)
Specifications
The Vascular Endothelial Growth Factor A (VEGFA) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VEGFA in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 98% homology of the sequence among different species, the kit can be used to detect mouse and rat samples.
Sensitivity:
5.8pg/ml
Range:
15.6-1000pg/ml
Specificity:
This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor A (VEGFA). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor A (VEGFA) and analogues was observed.
Precision:
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle:
The microplate provided in this kit has been pre-coated with an antibody specific to VEGFA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VEGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VEGFA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit Components:
96-well strip plate, 1x Plate
Standard, 2x vial
Detection Reagent A, 1×120ul
Detection Reagent B, 1×120ul
TMB Substrate, 1×9ml
Wash Buffer (30X), 1×20ml
Standard Diluent, 1×20ml
Assay Diluent A, 1×12ml
Assay Diluent B, 1×12ml
Stop Solution, 1×6ml
Storage and Stability:
Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.