VEGFR1/FLT-1 Protein, Human, Recombinant (His & Avi)
Référence NB-64-54194-200ug
Conditionnement : 200ug
Marque : Neo Biotech
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VEGFR1/FLT-1 Protein, Human, Recombinant (His & Avi)
(Synonyms: VEGFR1, VEGF R1, FRT, Flt-1, FLT1, FLT) Copy Product InfoSynonyms: VEGFR1, VEGF R1, FRT, Flt-1, FLT1, FLT
Catalog No. TMPK-00450 Copy Product Info
VEGFR1/FLT-1 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 85.1 kDa and the accession number is P17948-1.
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95% as determined by Bis-Tris PAGE
Appearance:Lyophilized powder
Color:White
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Product Information
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Human PGF, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA. |
| Description | VEGFR1/FLT-1 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 85.1 kDa and the accession number is P17948-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P17948-1 |
| Construction | Ser27-Asn756 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | VEGFR1, VEGF R1, FRT, Flt-1, FLT1, FLT |
| Research Background | VEGFR1 (vascular endothelial growth factor receptor 1), also called Flt-1 (Fms-like tyrosine kinase), is a 180 kDa type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTKs).yrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. |
| Molecular Weight | 85.1 kDa (predicted). Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Keywords
VEGFR-1VEGFR 1FLT 1
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Related Tags: VEGFR1/FLT-1 Protein, Human, Recombinant (His & Avi) chemical structure | VEGFR1/FLT-1 Protein, Human, Recombinant (His & Avi) molecular weight
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