Vimentin (FLJ36605, VIM)
Référence MBS6508452-0,04mg
Conditionnement : 0.04mg
Marque : MyBiosource
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Western Blot using MBS6508452
Formalin-fixed, paraffin-embedded human breast carcinoma. stained with MBS6508452.
Vimentin (FLJ36605, VIM) (VIM) Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6508452Vimentin is the main intermediate filament protein in mesenchymal cells and thus is of value in the differential identification of undifferentiated neoplasms. In tissue sections, this reagent shows positive reaction on connective tissue, endothelial cells,… More details
Product Overview
Online Datasheet in PDFProduct Name
Vimentin (FLJ36605, VIM), Monoclonal Antibody
Full Product Name
Vimentin (FLJ36605, VIM)
Product Gene Name
anti-VIM antibody
Product Synonym Names
Vimentin
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q1123
Host
Mouse
Species Reactivity
Bovine, Canine, Chicken, Feline, Human, Monkey, Mouse, Porcine, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Concentration
~1mg/ml (lot specific)
Specificity
Recognizes a 57-60kD protein, human Vimentin. Shows no crossreactivity with other closely related intermediate filament proteins including desmin and GFAP. Species Crossreactivity: monkey, bovine, canine, feline, chicken, porcine, mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
53,668 Da
Immunogen
Purified vimentin from porcine eye lens.
Positive Control
Breast carcinoma tissue, colon, prostate, tonsil, tonsilar lymphoma, uterus and ureter
Product Categories/Family for anti-VIM antibody
Antibodies-Primary; Antibodies-Extracellular Matrix Proteins
Applications Tested/Suitable for anti-VIM antibody
Immunochemistry (IC), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-VIM antibody
Western Blot: 1:500 detects Vitenmin on 10ug og HeLa lysates. HeLa lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with V2122-08. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunohistochemistry: 5-10ug/ml; Can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antibody activity.
Immunocytochemistry: 5-10ug/ml
Immunohistochemistry: 5-10ug/ml; Can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antibody activity.
Immunocytochemistry: 5-10ug/ml
Other Notes
Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for VIM purchase
MBS6508452 is an antibody targeting VIM. Also known as Vimentin (FLJ36605, VIM). Reactive in Bovine, Canine, Chicken, Feline, Human, Monkey, Mouse, Porcine, Rat. Host: Mouse.
Additional Details
NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
vimentin isoform X1
NCBI Official Symbol
VIM
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_PIG
NCBI Protein Information
vimentin
UniProt Comments for VIM
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Show more Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-VIM antibody
| Reference Product | PubMed Publications |
|---|---|
| CDH2 antibody | >12 publications with VIM and CDH2 |
| TGFB1 antibody | >12 publications with VIM and TGFB1 |
| NES antibody | >8 publications with VIM and NES |
| CASP3 antibody | >6 publications with VIM and CASP3 |
| STAT3 antibody | >4 publications with VIM and STAT3 |
| LGALS1 antibody | >2 publications with VIM and LGALS1 |
| AKT2 antibody | >1 publications with VIM and AKT2 |
Pathways associated with anti-VIM antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| Apoptotic Cleavage Of Cellular Proteins Pathway antibodies | Apoptotic Cleavage Of Cellular Proteins Pathway Diagram |
| Apoptotic Execution Phase Pathway antibodies | Apoptotic Execution Phase Pathway Diagram |
| Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies | Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram |
| Epstein-Barr Virus Infection Pathway antibodies | Epstein-Barr Virus Infection Pathway Diagram |
| Epstein-Barr Virus Infection Pathway antibodies | Epstein-Barr Virus Infection Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
| Muscle Contraction Pathway antibodies | Muscle Contraction Pathway Diagram |
| Programmed Cell Death Pathway antibodies | Programmed Cell Death Pathway Diagram |
Diseases associated with anti-VIM antibody
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >420 publications with VIM and Nervous System Diseases |
| Neurodegenerative Diseases Antibodies | >178 publications with VIM and Neurodegenerative Diseases |
| Inflammation Antibodies | >56 publications with VIM and Inflammation |
| Kidney Diseases Antibodies | >40 publications with VIM and Kidney Diseases |
| Pain Antibodies | >37 publications with VIM and Pain |
| Disease Models, Animal Antibodies | >36 publications with VIM and Disease Models, Animal |
| Hemorrhage Antibodies | >34 publications with VIM and Hemorrhage |
| Neoplasm Metastasis Antibodies | >33 publications with VIM and Neoplasm Metastasis |
| Liver Diseases Antibodies | >33 publications with VIM and Liver Diseases |
| Breast Neoplasms Antibodies | >31 publications with VIM and Breast Neoplasms |
Organs/Tissues associated with anti-VIM antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >505 publications with VIM and Brain |
| Blood Antibodies | >216 publications with VIM and Blood |
| Muscle Antibodies | >118 publications with VIM and Muscle |
| Kidney Antibodies | >60 publications with VIM and Kidney |
| Liver Antibodies | >57 publications with VIM and Liver |
| Bone Marrow Antibodies | >52 publications with VIM and Bone Marrow |
| Eye Antibodies | >48 publications with VIM and Eye |
| Lung Antibodies | >40 publications with VIM and Lung |
| Embryonic Tissue Antibodies | >35 publications with VIM and Embryonic Tissue |
| Skin Antibodies | >32 publications with VIM and Skin |