Specifications

Product Data
Locus ID	7490
Synonyms	AWT1; GUD; NPHS4; WAGR; WIT-2; WT33
Vector	pGFP-C-shLenti
E. coli Selection	Chloramphenicol (34 ug/ml)
Mammalian Cell Selection	Puromycin
Format	Lentiviral plasmids
Components	WT1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 7490). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_000378, NM_001198551, NM_001198552, NM_024424, NM_024425, NM_024426, NM_000378.1, NM_000378.2, NM_000378.3, NM_000378.4, NM_024426.1, NM_024426.2, NM_024426.3, NM_024426.4, NM_024424.1, NM_024424.3, NM_024425.1, NM_024425.2, NM_001198551.1, NM_001198552.1, BC032861, BC046461, NM_001367854, NR_160306, NM_001198552.2, NM_000378.6, NM_024426.5, NM_024424.5
UniProt ID	P19544
Summary	This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. provided by RefSeq, Mar 2015
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact tech@biovalley.fr. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage	Store at -20°C
Shipping	Ambient

Product Manuals

HuSH-29 shRNA Plasmids HuSH pRS Cloning Vector HuSH RNAi Validation Vector HuSH pGFP-V-RS Cloning Vector

FAQs

shRNA (HuSH-29)

SDS

SDS for TL300442

RNAi Resources

Product overview Video shRNA Selection Guide FAQ: shRNA FAQ: siRNA FAQ; miRNA Product Brochure: RNAi QC Data for Lenti shRNA