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> Wnt3a Protein, Mouse, Recombinant (His)

Wnt3a Protein, Mouse, Recombinant (His)

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Référence NB-64-51528-1mg

Conditionnement : 1mg

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Wnt3a Protein, Mouse, Recombinant (His)

(Synonyms: Wnt-3a, Wnt3a, Protein Wnt-3a) Copy Product Info

Synonyms: Wnt-3a, Wnt3a, Protein Wnt-3a

Catalog No. TMPH-02859 Copy Product Info
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro). Wnt3a Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 40.9 kDa and the accession number is P27467.
Other Forms of Wnt3a Protein, Mouse, Recombinant (His):
Wnt3a Protein, Human, Recombinant (His)TMPH-01961
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:90%
Appearance:Lyophilized powder
Color:White
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Product Information

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. For confirmed protein cellular activity, we recommend selecting TMPY-06987.
Description
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro). Wnt3a Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 40.9 kDa and the accession number is P27467.
Species
Mouse
Expression System
E. coli
TagN-10xHis
Accession NumberP27467
Amino AcidSYPIWWSLAVGPQYSSLSTQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGVKAGIQECQHQFRGRRWNCTTVSNSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSAAICGCSSRLQGSPGEGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARTERRREKCHCVFHWCCYVSCQECTRVYDVHTCK
Construction19-352 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Wnt3a Protein, Mouse, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationTris-based buffer, 50% glycerol
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsWnt-3a, Wnt3a, Protein Wnt-3a
Research Background
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).
Chemical Properties
Molecular Weight40.9 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Protein Wnt3a
Related Tags: Wnt3a Protein, Mouse, Recombinant (His) chemical structure | Wnt3a Protein, Mouse, Recombinant (His) molecular weight