XRCC6 (X-ray Repair Cross-complementing Protein 6, 5'-deoxyribose-5-phosphate Lyase Ku70, 5'-dRP Lyase Ku70, 70kD Subunit of Ku Antigen, ATP-dependent DNA Helicase 2 Subunit 1, ATP-dependent DNA Helicase II 70kD Subunit, CTC box-binding Factor 75kD Subunit, CTC75, CTCBF, DNA Repair Protein XRCC6, Lupus Ku Autoantigen Protein p70, Ku70, Thyroid-lupus Autoantigen, TLAA, X-ray Repair Complementing Defective Repair in Chinese Hamster Cells 6, G22P1) (Biotin)

The Ku heterodimer comprised of Ku70 and Ku80 subunits is an ATP-dependent DNA helicase II (Lupus Ku autoantigen), involved in nonhomologous end-joining (NHEJ) in mammalian cells. It also plays an important role in V (D) J recombination, apoptosis, telomere fusion, and double-strand break repair, thus contributing to genomic stability. It recruits a 465kD DNA-dependent protein kinase catalytic subunit (DNA-PK) to the DNA double-strand break sites stimulating the DNA-PK kinase activity. Ku70 is the 70kD subunit of the Ku heterodimer that is mainly responsible for the DNA binding activity. It contains two DNA-binding domains, one is located at the N terminus before residue 440, and the other one is located at the C terminus. The DNA binding activity of the C-terminal DNA-binding domain is independent of the heterodimer formation with Ku80, whereas the DNA binding activity of the N-terminal domain of Ku70 depends on heterodimer formation with Ku80. It interacts with Bax and blocks the mitochondrial translocation of Bax. Large amounts of autoantibodies to Ku70 are observed in patients suffering form systemic lupus erythematosus (SLE) and related disorders.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

AA Sequence:
MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.  For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

Note: Applications are based on unconjugated antibody.