Orb1088185-24 | Zebrafish BDNF(Brain Derived Neurotrophic Factor)

Zebrafish BDNF(Brain Derived Neurotrophic Factor) ELISA Kit

Catalog Number	orb1088185
Category	Assays and Kits
Description	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor(BDNF). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor(BDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Derived Neurotrophic Factor(BDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Derived Neurotrophic Factor(BDNF) in the samples is then determined by comparing the OD of the samples to the standard curve.
Reactivity	Zebrafish
Range	7.82-500 pg/mL
Sensitivity	3.1 pg/mL
Protein Length	3.5h
Alternative names	Neurotrophin, Abrineurin
Note	For research use only
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration Date	Please enquire.