Orb1088148-24 | Zebrafish HSP-70/HSPA9(HeatShock Protein 70) ELISA

Zebrafish HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit

Catalog Number	orb1088148
Category	Assays and Kits
Description	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HeatShock Protein 70(HSP-70/HSPA9). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HeatShock Protein 70(HSP-70/HSPA9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HeatShock Protein 70(HSP-70/HSPA9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HeatShock Protein 70(HSP-70/HSPA9) in the samples is then determined by comparing the OD of the samples to the standard curve.
Reactivity	Zebrafish
Range	3.13-200 ng/mL
Sensitivity	1.27 ng/mL
Protein Length	3.5h
Alternative names	DnaK, 70 Kilodalton Heat Shock Proteins, Heat shoc
Note	For research use only
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration Date	Please enquire.