Orb1736541-48 | Zebrafish IgM(Immunoglobulin M) ELISA Kit Biovalley

Zebrafish IgM(Immunoglobulin M) ELISA Kit [Out of stock]

Catalog Number	orb1736541
Category	Tools
Description	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish IgM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish IgM in the samples is then determined by comparing the OD of the samples to the standard curve.
Range	0.78-50 μg/mL
Sensitivity	0.21 μg/mL
Alternative names	IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chai
Note	For research use only
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration Date	12 months from date of receipt.