This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish NE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish NE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish NE in the samples is then determined by comparing the OD of the samples to the standard curve.
Species/Host
Zebrafish
Reactivity
Zebrafish
Range
78.13-5000 pg/mL
Sensitivity
26.1 pg/mL
Alternative names
Noradrenaline
Note
For research use only
Sample Types
serum, plasma, cell lysates, cell culture supernates and other biological fluids