Zebrafish Talin (TLN) ELISA Kit
Référence MBS2000268-5x96
Conditionnement : 5x96-Strip-Wells
Marque : MyBiosource
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Talin (TLN) ELISA Kit for Zebrafish
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2000268SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Talin (TLN). Standards or samples are then added to the appropriate microtiter plate wells with a… More details
Product Overview
Online Datasheet in PDFProduct Name
Talin (TLN), ELISA Kit
Full Product Name
Zebrafish Talin (TLN) ELISA Kit
Product Gene Name
TLN elisa kit
Product Synonym Names
ELISA Kit for Talin (TLN)
Species Reactivity
Zebrafish
Assay Type
Sandwich
Samples
Serum, plasma and other biological fluids
Detection Range
0.781-50 ng/mL The standard curve concentrations used for the ELISA's were 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL, 1.563 ng/mL, 0.781 ng/mL
Sensitivity
Typically less than 0.195 ng/mL.
Specificity
This assay has high sensitivity and excellent specificity for detection of Talin (TLN).
No significant cross-reactivity or interference between Talin (TLN) and analogues was observed.
No significant cross-reactivity or interference between Talin (TLN) and analogues was observed.
Preparation and Storage
For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Talin (TLN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Talin (TLN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Talin (TLN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of TLN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TLN purchase
MBS2000268 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Talin (TLN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TLN. The ELISA analytical biochemical technique of the MBS2000268 kit is based on TLN antibody-TLN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TLN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TLN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for TLN. It may not necessarily be applicable to this product.
NCBI Official Full Name
talin
NCBI Summary for TLN
This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]
Show more Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with TLN elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Activation Of Chaperone Genes By XBP1(S) Pathway antibodies | Activation Of Chaperone Genes By XBP1(S) Pathway Diagram |
| Activation Of Chaperones By IRE1alpha Pathway antibodies | Activation Of Chaperones By IRE1alpha Pathway Diagram |
| Axon Guidance Pathway antibodies | Axon Guidance Pathway Diagram |
| Developmental Biology Pathway antibodies | Developmental Biology Pathway Diagram |
| EGF Receptor (ErbB1) Signaling Pathway antibodies | EGF Receptor (ErbB1) Signaling Pathway Diagram |
| Focal Adhesion Pathway antibodies | Focal Adhesion Pathway Diagram |
| Focal Adhesion Pathway antibodies | Focal Adhesion Pathway Diagram |
| Focal Adhesion Pathway antibodies | Focal Adhesion Pathway Diagram |
| GRB2:SOS Provides Linkage To MAPK Signaling For Intergrins Pathway antibodies | GRB2:SOS Provides Linkage To MAPK Signaling For Intergrins Pathway Diagram |
| HTLV-I Infection Pathway antibodies | HTLV-I Infection Pathway Diagram |