Zidovudine [30516-87-1]
Référence HY-17413-1ml
Conditionnement : 10mM/1mL
Marque : MedChemExpress
Zidovudine is a nucleoside reverse transcriptase inhibitor (NRTI), widely used to treat HIV infection. Zidovudine increases CRISPR/Cas9-mediated editing frequency.
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Zidovudine Chemical Structure
CAS No. : 30516-87-1
This product is a controlled substance and not for sale in your territory.
Based on 6 publication(s) in Google Scholar
Other Forms of Zidovudine:
- Zidovudine-d3 Obtenir un devis
- Zidovudine-13C,d3 Obtenir un devis
- Zidovudine-d4 Obtenir un devis
Description |
Zidovudine is a nucleoside reverse transcriptase inhibitor (NRTI), widely used to treat HIV infection. Zidovudine increases CRISPR/Cas9-mediated editing frequency. |
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IC50 & Target[1][2] |
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In Vitro |
Zidovudine inhibits SVG, Primary human fetal astrocytes (PFA), peripheral blood mononuclear cells (PBMC), and monocyte-derived macrophages (MDM) with EC50 of 17, 1311, 8, and 5 nM, respectively. Zidovudine inhibits SVG, PFA, PBMC, and MDM with EC90 of 0.205 μM, 44.157 μM, 0.481 μM, and 0.219 μM, respectively[1]. Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise, targeted changes to the genome of living cells. CXCR4 is a co-receptor for the human immunodeficiency virus type 1 (HIV-1) infection and has been considered as an important therapeutic target for AIDS. CXCR4 mediates viral entry into human CD4+ cells by binding to envelope protein, gp120. Human CXCR4 gene is efficiently disrupted by CRISPR/Cas9-mediated genome editing, leading to HIV-1 resistance of human primary CD4+ T cells. The Cas9-mediated ablation of CXCR4 demonstrated high specificity and negligible off-target effects without affecting cell division and propagation[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
Intravitrous injection of the NRTIs Lamivudine (3TC), Zidovudine (AZT), or Abacavir (ABC) suppresses the laser-induced choroidal neovascularization (CNV) in wild-type mice compared to PBS vehicle. The mean level of VEGF-A in the RPE/choroid, which peaks on day 3 after laser injury, is significantly reduced in 3TC-, AZT- and ABC-treated eyes compared with control eyes in wild-type mice, but not inP2rx7-/- mice[3]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Essai clinique |
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Masse moléculaire |
267.24 |
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Formule |
C10H13N5O4 |
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CAS No. |
30516-87-1 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
OC[C@@H]1[C@@H](N=[N+]=[N-])C[C@H](N2C(NC(C(C)=C2)=O)=O)O1 |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
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Solvant et solubilité |
In Vitro:
DMSO : ≥ 100 mg/mL (374.20 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 16.67 mg/mL (62.38 mM; ultrasonic and warming and heat to 60°C) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Pureté et documentation |
Purity: 99.93% |
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Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO | 1 mM | 3.7420 mL | 18.7098 mL | 37.4195 mL | 93.5489 mL |
5 mM | 0.7484 mL | 3.7420 mL | 7.4839 mL | 18.7098 mL | |
10 mM | 0.3742 mL | 1.8710 mL | 3.7420 mL | 9.3549 mL | |
15 mM | 0.2495 mL | 1.2473 mL | 2.4946 mL | 6.2366 mL | |
20 mM | 0.1871 mL | 0.9355 mL | 1.8710 mL | 4.6774 mL | |
25 mM | 0.1497 mL | 0.7484 mL | 1.4968 mL | 3.7420 mL | |
30 mM | 0.1247 mL | 0.6237 mL | 1.2473 mL | 3.1183 mL | |
40 mM | 0.0935 mL | 0.4677 mL | 0.9355 mL | 2.3387 mL | |
50 mM | 0.0748 mL | 0.3742 mL | 0.7484 mL | 1.8710 mL | |
60 mM | 0.0624 mL | 0.3118 mL | 0.6237 mL | 1.5591 mL | |
DMSO | 80 mM | 0.0468 mL | 0.2339 mL | 0.4677 mL | 1.1694 mL |
100 mM | 0.0374 mL | 0.1871 mL | 0.3742 mL | 0.9355 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.