Kits and columns for ion exchange chromatography
Ion exchange chromatography (IEX) separates proteins depending on their net surface charge, through electrostatic interactions that occur between proteins and a stationary charged phase. Two types of IEX exist:
- Anion exchange (positively charged stationary phase which binds to negatively charged proteins)
- Cation exchange (Negatively charged stationary phase which binds to positively charged proteins)
Ion exchange chromatography is commonly used as an intermediate step in a protein purification method, but it can give high resolution for some proteins when used earlier or later during purification.
All proteins have a net charge which depends on the amino acid composition of the protein as well as covalently attached modifications. The net charge of a protein is influenced by the pH of the solvent in which it is dissolved, as the solvent exchanges hydrogen ions with the proteins. The isoelectric point (pI) of a protein is the pH at which the protein has no net charge. At pH above pH, a protein will have a net negative charge, while a pH below the pH will lead to a net positive charge. Thus, the pH of the solvent can be adjusted to facilitate binding to IEX or promote elution of a bound protein.
Frequently Asked Questions (FAQ)
Where can I find ion exchange (iex) products on the Biovalley website?
ion exchange (iex) products are available in this section and can be sorted by product families or sub-categories, such as Detergent removal. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the ion exchange (iex) category?
The ion exchange (iex) category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Detergent removal sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a ion exchange (iex) product?
To find a ion exchange (iex) product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Biovalley stock several product codes in the ion exchange (iex) category?
Yes, Biovalley distributes several product references in the ion exchange (iex) category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for ion exchange (iex) products?
Yes, you can request a quote for ion exchange (iex) products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a ion exchange (iex) product is available to order?
The availability of a ion exchange (iex) product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.
What should I do if I cannot find the ion exchange (iex) product I am looking for?
If the ion exchange (iex) product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all ion exchange (iex) products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.