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> 1-Desamino 8D Arginine Vasopressin ELISA Kit

1-Desamino 8D Arginine Vasopressin ELISA Kit

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Cat# OKCD02265

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for 1-Desamino 8D Arginine Vasopressin ELISA Kit (OKCD02265)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)83-94%99-105%96-103%83-101%
EDTA plasma(n=5)83-90%82-89%78-99%96-103%
heparin plasma(n=5)88-95%80-91%78-102%78-89%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1-Desamino 8D Arginine Vasopressin (DDAVP) and unlabeled 1-Desamino 8D Arginine Vasopressin (DDAVP) (Standards or samples) with the pre-coated antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample.
ELISA Kit Range246.9-20,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)84-105102
EDTA plasma(n=5)90-10193
heparin plasma(n=5)78-9683
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 1-Desamino 8D Arginine Vasopressin (DDAVP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 1-Desamino 8D Arginine Vasopressin (DDAVP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-1-Desamino 8D Arginine Vasopressin Microplate96 Wells (12 x 8 Well Strips)
1-Desamino 8D Arginine Vasopressin Lyophilized Standard2
100X 1-Desamino 8D Arginine Vasopressin Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Additional InformationCAS: 16679-58-6
PubChem: 5311065
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity39.5 pg/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolDDAVP
Alias SymbolsDESMOPRESSIN; 16679-58-6; DDAVP; UNII-ENR1LLB0FP
Protein Name1-Desamino 8D Arginine Vasopressin
Description of TargetDesmopressin is a Factor VIII Activator and Vasopressin Analog. The physiologic effect of desmopressin is by means of Increased Coagulation Factor VIII Activity and Increased Coagulation Factor VIII Concentration. The chemical classification of desmopressin is Vasopressins.