Butyric Acid ELISA Kit
Cat# OKCD02255
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for Butyric Acid ELISA Kit (OKCD02255) |
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| COA Datasheet | ClickHereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 2h | ||||||||||||||||||||||
| ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Butyric Acid (BA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Butyric Acid (BA) and unlabeled Butyric Acid (BA) (Standards or samples) with the pre-coated antibody specific to Butyric Acid (BA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Butyric Acid (BA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Butyric Acid (BA) in the sample. | ||||||||||||||||||||||
| ELISA Kit Range | 123.5-10,000pg/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Butyric Acid (BA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Butyric Acid (BA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | CAS: 107-92-6 PubChem: 264 | ||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Biological agents | ||||||||||||||||||||||
| Sensitivity | 51.5 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
| Gene Symbol | BA |
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| Alias Symbols | Butyric acid; Butanoic acid; N-Butyric acid; N-Butanoic acid; Propylformic acid |
| Protein Name | Butyric Acid |
| Description of Target | Butyric Acid is a saturated short-chain fatty acid with a 4-carbon backbone. Butyric acid is commonly found in esterified form in animal fats and plant oils. |