Carnosine ELISA Kit

Cat# OKCD02258

Size : 96Wells

Brand : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Carnosine ELISA Kit (OKCD02258)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)87-95%94-103%93-105%98-105%
EDTA plasma(n=5)80-94%93-102%86-104%93-104%
heparin plasma(n=5)88-101%78-101%78-89%82-99%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Carnosine (Car) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Carnosine (Car) and unlabeled Carnosine (Car) (Standards or samples) with the pre-coated antibody specific to Carnosine (Car). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Carnosine (Car) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Carnosine (Car) in the sample.
ELISA Kit Range11.11-900ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)93-105101
EDTA plasma(n=5)85-9289
heparin plasma(n=5)88-9792
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carnosine (Car) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carnosine (Car) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Carnosine Microplate96 Wells (12 x 8 Well Strips)
Carnosine Lyophilized Standard2
Carnosine Biotin Complex1
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Biotin Complex Reconstitution Buffer1 x 300 uL
Additional InformationCAS: 305-84-0
PubChem: 439224
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity3.53 ng/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolCAR
Alias SymbolsL-Carnosine; Carnosine; Beta-Alanyl-L-histidine; 305-84-0; Ignotine
Protein NameCarnosine
Description of TargetL-Carnosine is a naturally occurring dipeptide neuropeptide found in muscles.