Docosahexaenoic Acid ELISA Kit

Cat# OKCD02266

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Docosahexaenoic Acid ELISA Kit (OKCD02266)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)79-90%91-101%97-105%96-103%
EDTA plasma(n=5)92-102%88-104%83-101%81-95%
heparin plasma(n=5)83-90%79-97%79-91%93-104%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Docosahexaenoic Acid (DHA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Docosahexaenoic Acid (DHA) and unlabeled Docosahexaenoic Acid (DHA) (Standards or samples) with the pre-coated antibody specific to Docosahexaenoic Acid (DHA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Docosahexaenoic Acid (DHA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Docosahexaenoic Acid (DHA) in the sample.
ELISA Kit Range12.35-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)84-10188
EDTA plasma(n=5)86-10194
heparin plasma(n=5)83-9389
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Docosahexaenoic Acid (DHA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Docosahexaenoic Acid (DHA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Docosahexaenoic Acid Microplate96 Wells (12 x 8 Well Strips)
Docosahexaenoic Acid Lyophilized Standard2
100X Docosahexaenoic Acid Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Additional InformationCAS: 6217-54-5
PubChem: 445580
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 5.51 pg/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolDHA
Alias SymbolsDocosahexaenoic acid; Doconexent; Cervonic acid; Doconexento; Doconexentum
Protein NameDocosahexaenoic Acid
Description of TargetDocosahexaenoic Acid n-3 is a polyunsaturated very long-chain fatty acid with a 22-carbon backbone and 6 double bonds, originating from the 3rd, 6th, 9th, 12th, 15th and 18th positions from the methyl end.