Sulfonamides residues ELISA Kit
Cat# OKAO00112
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for Sulfonamides residues ELISA Kit (OKAO00112) |
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| Predicted Species Reactivity | General | ||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Sulfonamides residues ELISA Kit (OKAO00112) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Sulfonamides residues has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Sulfonamides residues antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample. | ||||||||||||||||||||||
| ELISA Kit Range | 0.5-40.5 ppb | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40) | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Tissue (meat, chicken, duck, liver, shrimp, fish, egg), Serum, honey, Urine, milk | ||||||||||||||||||||||
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| Publications | Engineering of Organic Solvent-Tolerant Antibody to Sulfonamides by CDR Grafting for Analytical Purposes. Anal Chem. (2021). 33728902 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Competitive Inhibition ELISA | ||||||||||||||||||||||
| Description |
| Alias Symbols | Sas |
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| Protein Name | Sulfonamides residues |
| Description of Target | The related sulfinamides (R(S=O)NHR) are amides of sulfinic acids (R(S=O)OH) (see sulfinyl). Chiral sulfinamides such as tert-butanesulfinamide, p-toluenesulfinamide and 2,4,6-trimethylbenzenesulfinamide are relevant to asymmetric synthesis. |