Vitamin D3 ELISA Kit

Cat# OKEH02571

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02571
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Vitamin D3 ELISA Kit (OKEH02571) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Vitamin D3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Vitamin D3 and incubated. The Vitamin D3 found in the sample or standards competes with the biotinylated Vitamin D3 for limited binding sites on the immobilized anti-Vitamin D3 antibody. Excess unbound biotinylated Vitamin D3 and sample or standard Vitamin D3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Vitamin D3 captured in the well and inversely proportional to the amount of Vitamin D3 which was contained in the sample or standard.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Vitamin D3 Microplate96 Wells (12 x 8 Well strips)
Vitamin D3 Lyophilized Standard2 x 100 ng
Sample Diluent1 x 20 mL
100X Vitamin D3-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: VD3
Target Name: Vitamin D3
::Pubchem: 50990069
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.4 ng/mL
SpecificityNatural and recombinant General Vitamin D3
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsVitamin D3, VD3, Cholecalciferol
Protein NameVitamin D3
Description of TargetCholecalciferol is a form of vitamin D, also called vitamin D3. Vitamin D3 has several forms: Cholecalciferol, (sometimes called calciol) is an inactive, unhydroxylated form of vitamin D3; Calcifediol (also called calcidiol, hydroxycholecalciferol, 25-hydroxyvitamin D3, etc. and abbreviated 25(OH)D) is one of the forms measured in the blood to assess vitamin D status; Calcitriol (also called 1,25-dihydroxyvitamin D3) is the active form of D3.