Recombinant protein corresponding to aa3360--3526 with an N-Terminal His-Tag from mouse APOB, expressed in E. coli. Swiss/UniProt Accession: E9Q414

Amino Acid Sequence:
H FLSSSSFVTD ALQYKLEGTS RLMRKRGLKL ATAVSLTNKF VKGSHDSTIS LTKKNMEASV RTTANLHAPI FSMNFKQELN GNTKSKPTVS SSIELNYDFN SSKLHSTATG GIDHKFSLES LTSYFSIESF TKGNIKSSFL SQEYSGSVAN EANVYLNSKG TRSSVR

Predicted Isoelectric Point:
9.8

Predicted Molecular Weight:
21.4kD

Accurate Molecular Weight:
21kD as determined by SDS-PAGE reducing conditions

Subcellular Location:
Secreted

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE.
Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.