Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. Alkaline phosphatase (AP) is conjugated to Arachis hypogaea (Peanut) Lectin (PNA) to show the binding of PNA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Alkaline phosphatase (AP) is conjugated to Arachis hypogaea (Peanut) Lectin (PNA) to show the binding of PNA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Recommended Usage: Recommended dilution is 0.5 – 10 µg/ml in 20mM Tris-HCl pH 7.5 containing 1mM CaCl2 and 0.5 mM MnCl2, 50 mM NaCl. Less than 0.2 mg/ml will agglutinate neuraminidase treated human erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Blotting, Glycobiology, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, ELLA/ELISA, Blotting

References

1.Jie Sun, Qing-li Yang*, jie Bi, Chu-shu Zhang, Li-na Yu and Feng Zhu. Purification and Identification of a Natural Lectin from the Seed of Peanut Arachis hypogaea. The Open Materials Science Journal, 2011, 5, 78-82
2.Erber, W. N., Asbahr, H., Meyer, B., Herrmann, R. P., & Davies, J. M. (1992). Peanut agglutinin (lectin from Arachis hypogaea) binding to hemopoietic cells: an immunophenotypic study using a biotin streptavidin technique. Pathology, 24(3), 173–176. https://doi.org/10.3109/00313029209063168.
3.Jones, C. J., Benbow, E. W., Knox, W. F., & Stoddart, R. W. (1988). Method for showing human spermatogenesis using Arachis hypogaea (AHA) lectin. Journal of clinical pathology, 41(9), 1017–1018. https://doi.org/10.1136/jcp.41.9.1017
4.Rambihar, C., & Kerman, K. (2010). Magnetic bead-based fluorometric detection of lectin-glycoprotein interactions. Talanta, 81(4-5), 1676–1680. https://doi.org/10.1016/j.talanta.2010.03.021