Dimethylsulfoxide (DMSO) for Cell Culture
Cat# D8050-01-100ml
Size : 100ml
Brand : US Biological
D8050-01 Dimethylsulfoxide (DMSO) for Cell Culture
Clone Type
PolyclonalGrade
Cell Culture GradeShipping Temp
RTStorage Temp
RTReady-to-use sterile filtered product conveniently packaged in septum screw-capped amber bottles. ||DMSO has been used as a cryoprotectant and is still an important constituent of cryoprotectant vitrification mixtures used to preserve organs, tissues, and cell suspensions. Without it, up to 90% of frozen cells will become inactive. It is particularly important in the freezing and long-term storage of embryonic stem cells and hematopoietic stem cells, which are often frozen in a mixture of 10% DMSO, a freezing medium, and 30% fetal bovine serum. In the cryogenic freezing of heteroploid cell lines (MDCK, VERO, etc.) a mixture of 10% DMSO with 90% EMEM (70% EMEM + 30% fetal bovine serum + antibiotic mixture) is used.||Synonyms:|Methyl Sulfoxide; Dimethylsulfoxide; DMSO||CAS No:|67-68-5||Molecular Formula:|C2H6SO||Molecular Weight:|78.13||Purity (GC):|≥99%||Specific Gravity: |1.095-1.097||Congealing Temp: |18.3°C||Refractive Index: |1.4755-1.4775||Water:|≤0.1%||Titratable Acid: |≤5ml||Residue after Evaporation: |≤0.01%||Absorbance: |To Pass ACS Specifications||Substances Darkened by KOH: |To Pass ACS Specifications||RNase: |None Detected ||DNase: |None Detected||Endotoxin: |None Detected||Note: DMSO will congeal easily and may remain a solid at cool temperatures. Warm slightly to return to liquid state.