Reagents and instruments for immunology, cell biology and molecular biology.
 
> F(ab')2 Rat IgM (mu chain) Antibody Phycoerythrin conjugated

F(ab')2 Rat IgM (mu chain) Antibody Phycoerythrin conjugated

Print Print
Please log in

Cat# orb348349-1ml

Size : 1ml

Brand : Biorbyt

Contact local distributor :


Phone : +1 850 650 7790

F(ab')2 Rat IgM (mu chain) Antibody Phycoerythrin conjugated

SKU: orb348349
This product can be custom made

Description

F(ab')2 Rat IgM (mu chain) antibody (RPE)

Images & Validation

−
F(ab')2 Rat IgM (mu chain) Antibody Phycoerythrin conjugated
Tested ApplicationsFC, IF
Dilution RangeFC: 1:100 - 1:250, IF: 1:100 - 1:250
ReactivityRat
Application Notes
F(ab')2 Anti-Rat IgM Antibody Phycoerythrin conjugation is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Key Properties

−
Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG F(ab')2
ImmunogenRat IgM whole molecule
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgM, and Rat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). No reaction was observed against other rat heavy or light chain proteins.
ConjugationRPE

Storage & Handling

−
StorageStore vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration0.5 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

−
Goat F(ab')2 Anti-Rat IgM (mu chain) Phycoerythrin Conjugated Antibody, Goat Fab2 Anti-Rat IgM mu chain Antibody PE Conjugation

Protocol Information

FC
Flow Cytometry
View Protocol