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> Human VEGF-A ELISA Kit

Human VEGF-A ELISA Kit

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Cat# ELH-VEGF-1

Size : 1PlateKit

Brand : RayBiotech

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Phone : +1 850 650 7790

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Human VEGF-A ELISA Kit Standard CurveHuman VEGF-A ELISA KitHuman VEGF-A ELISA KitHuman VEGF-A ELISA KitHuman VEGF-A ELISA KitHuman VEGF-A ELISA KitELH-VEGF was used to measure VEGFA levels in culture supernatants from normal colonic epithelial and colorectal cancer cell lines infected with Enterococcus faecalis. Figure adapted from Zhang et al., BMC Infectious Diseases, 2024.ELH-VEGF was used to measure VEGF-α in HBMSC/HUVEC co-culture supernatants treated with fluorinated hydroxyapatite extracts. Figure adapted from Wu et al., Bioengineering, 2024.ELH-VEGF was used to measure VEGFA in cell culture supernatants from A549 cells, normal fibroblasts (NFs), and cancer-associated fibroblasts (CAFs). Figure adapted from Chen et al., International Journal of Biological Sciences, 2023.ELH-VEGF was used to measure VEGFA in colorectal cancer cell culture supernatants following biliverdin treatment. Figure adapted from Zhang et al., Journal of Translational Medicine, 2023.ELH-VEGF was used to measure VEGFA secretion in papillary thyroid cancer (PTC) cell supernatants following modulation of MFSD4A-AS1 expression. Figure adapted from Liu et al., Endocrine-Related Cancer, 2023.ELH-VEGF was used to measure VEGFA in endothelial cell supernatants from HUVECs and myeloma-associated endothelial cells (MMECs). Figure adapted from Sun et al., Scientific Reports, 2023.Human VEGF-A ELISA KitHuman VEGF-A ELISA KitHuman VEGF-A ELISA Kit5 Plate Kit Packaging (one box)

Human VEGF-A ELISA Kit Standard Curve

Human VEGF-A ELISA Kit

RayBio® Human VEGF-A ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
10 pg/ml

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Detection Range
10 pg/ml - 6000 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)
2 - 5 fold
Accession Number
P15692
Gene Id
7422
Gene Symbols
VEGFA, VEGF
Protein Name / Synonyms
Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaAngiogenesis, Cardiovascular Disease, Antibody Drug Development, Protein Therapeutics, Vascular Biology
Shipping TypeBlue ice
Storage≤-20°C

Target Background

VEGF is a member of the PDGF/VEGF growth factor family that is often found as a disulfide linked homodimer. It is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in VEGF gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Human VEGF ELISA Kit Standard Curves

Human VEGF ELISA Dilution Series Diagram

Spiking & Recovery Results

VEGF ELISA Spiking and Recovery Results

Linearity Results

VEGF ELISA Linearity Results

Typical Data

ELH-VEGF-1 was used to measure VEGFA levels in culture supernatants from normal colonic epithelial and colorectal cancer cell lines infected with Enterococcus faecalis. Figure adapted from Zhang et al., BMC Infectious Diseases, 2024.

ELH-VEGF was used to measure VEGF-α in HBMSC/HUVEC co-culture supernatants treated with fluorinated hydroxyapatite extracts. Figure adapted from Wu et al., Bioengineering, 2024.

ELH-VEGF was used to measure VEGFA in cell culture supernatants from A549 cells, normal fibroblasts (NFs), and cancer-associated fibroblasts (CAFs). Figure adapted from Chen et al., International Journal of Biological Sciences, 2023.

ELH-VEGF was used to measure VEGFA in colorectal cancer cell culture supernatants following biliverdin treatment. Figure adapted from Zhang et al., Journal of Translational Medicine, 2023.

ELH-VEGF was used to measure VEGFA secretion in papillary thyroid cancer (PTC) cell supernatants following modulation of MFSD4A-AS1 expression. Figure adapted from Liu et al., Endocrine-Related Cancer, 2023.

ELH-VEGF was used to measure VEGFA in endothelial cell supernatants from HUVECs and myeloma-associated endothelial cells (MMECs). Figure adapted from Sun et al., Scientific Reports, 2023.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µL to 1 µL volumes
  • Adjustable 1-25 µL pipettes for reagent preparation
  • 100 µL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µL of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

citations

  • The pathogenicity of vancomycin-resistant Enterococcus faecalis to colon cancer cells

    Year:
    2024
    Journal Name:
    Bmc Infectious Diseases
    PubMed ID:
    38378500

  • Serum VEGF-A levels on admission in COVID-19 patients correlate with SP-D and neutrophils, reflecting disease severity: A prospective study

    Year:
    2024
    Journal Name:
    Cytokine
    PubMed ID:
    38554499

  • Heparan Sulfate-Collagen Surface Multilayers Support Serum-Free Microcarrier Culture of Mesenchymal Stem Cells

    Year:
    2024
    Journal Name:
    ACS Biomater Sci Eng
    PubMed ID:
    39187752

  • Fluorinated Porcine Bone-Derived Hydroxyapatite Promotes Vascularized Osteogenesis by Coordinating Human Bone Marrow Mesenchymal Stem Cell/Human Umbilical Vein Endothelial Cell Complexes

    Year:
    2024
    Journal Name:
    Bioengineering (Basel, Switzerland)
    PubMed ID:
    39768105

  • Distribution of Bevacizumab into the Cerebrospinal Fluid of Children and Adolescents with Recurrent Brain Tumors

    Year:
    2024
    Journal Name:
    Paediatr Drugs
    PubMed ID:
    38587585

  • Correlation between vascular endothelial growth factor, soluble urokinase plasminogen activator receptor, and tricuspid annular plane systolic excursion/systolic pulmonary artery pressure ratio in group E chronic obstructive lung disease

    Year:
    2024
    Journal Name:
    Rev Assoc Med Bras (1992)
    PubMed ID:
    39536250

  • Cancer-associated fibroblasts promote migration and invasion of non-small cell lung cancer cells via METTL3-mediated RAC3 m6A modification

    Species:
    Human
    Sample Type:
    A549 cells, normal fibroblasts (NFs), Cancer-associated fibroblasts (CAFs)
    Year:
    2023
    Journal Name:
    International Journal of Biological Sciences

  • Design of Blood Vessel Models using Magnetic-Responsive Vascular Platforms

    Species:
    Human
    Sample Type:
    human umbilical vein endothelial cells (HUVECs)
    Year:
    2023
    Journal Name:
    Advanced Materials Technologies

  • Enterococcus faecalis promotes the progression of colorectal cancer via its metabolite: biliverdin

    Species:
    Human
    Sample Type:
    HCT116 cell, SW480 cell, SW620 cell
    Year:
    2023
    Journal Name:
    Journal of Translational Medicine

  • Long non-coding RNA MFSD4A-AS1 promotes lymphangiogenesis and lymphatic metastasis of papillary thyroid cancer

    Species:
    Human
    Sample Type:
    B-CPAP cell, K1 cell
    Year:
    2023
    Journal Name:
    Endocrine-related Cancer

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