Immunohistochemistry (Frozen sections)
Immunohistochemistry (Frozen sections) using Myod1 monoclonal antibody, clone 5.8A (Cat # MAB0077) monoclonal antibody. A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent) .
Western Blot (Cell lysate)
Western blot analysis for Myod1 expression in various small round cell tumor lines using 1 ug/mL Myod1 monoclonal antibody, clone 5.8A (Cat # MAB0077) monoclonal antibody. The antibody only reacts with a band of approx. 45 kD in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines.
- Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant Myod1.
Immunogen
Recombinant protein corresponding to amino acids 180-189 of mouse Myod1.
Host
Mouse
Reactivity
Cat, Human, Mouse, Rat
Form
Liquid
Isotype
IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Frozen sections) using Myod1 monoclonal antibody, clone 5.8A (Cat # MAB0077) monoclonal antibody. A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent) .Immunofluorescence
Immunocytochemistry
Immunoprecipitation
Western Blot (Cell lysate)
Western blot analysis for Myod1 expression in various small round cell tumor lines using 1 ug/mL Myod1 monoclonal antibody, clone 5.8A (Cat # MAB0077) monoclonal antibody. The antibody only reacts with a band of approx. 45 kD in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines. - Gene Info — Myod1
- Publication Reference
- Skeletal muscle insulin resistance induced by adipocyte-conditioned medium: underlying mechanisms and reversibility.
Sell H, Eckardt K, Taube A, Tews D, Gurgui M, Van Echten-Deckert G, Eckel J.
American Journal of Physiology. Endocrinology and Metabolism 2008 Mar; 294(6):E1070.
- Skeletal muscle insulin resistance induced by adipocyte-conditioned medium: underlying mechanisms and reversibility.