Screen the phosphorylation and total levels of 500 human proteins. Suitable for serum, plasma, cell culture supernatants, and lysates.
Lead Time: 1-2 business days
High Density
Phosphorylation Screening Array
Phosphorylation Screening Array
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 500 |
| Solid Support | Glass Slide |
| Design Principle | Label-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Post-Translational Modifications, Phosphorylation |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- High density arrays
- Unbiased detection through direct biotin labeling of samples
- Low sample consumption
- High detection sensitivity
- Same day result
- Affordable and simple to use
Target Names
ABL2, ACTB, ACTN1, Akt1, ALK, Annexin A1, Annexin A2, ARAF, ATF2, ATM, ATR, AURKA, AURKB, AURKC, AXL, BCR, Blk, BMPR-II, BRAF, BRD2, BRSK1, Btk, BUB1B, C9orf78, c-Abl, CAMK1, CAMK1D, CAMK2A, CAMK4, CAMKK1, CAVIN1, CDC7, CDK1, CDK10, CDK11A, CDK12, CDK16, CDK17, CDK2, CDK4, CDK5, CDK6, CDK7, CDK9, CDKL5, Chk1, Chk2, CHUK, CLK1, CREB1, Csk, CSNK2A1, DDR1, DDR2, DNA-PKcs, DOK3, DYRK1A, DYRK2, EEF1A1, EEF2, EGFR, EIF2AK2, EIF2AK3, EIF4EBP1, EML4, ENO1, EphA1, EphA2, EphA3, EphB1, EphB2, EphB3, EphB4, ErbB2, ErbB3, ErbB4 / HER4, Erbin, ESYT1, FAK, FER, Fes, FGF R3, FGFR1, FGFR2, Fgr, Flt-3 / Flk-2, FRK, Fyn, G6PD, GAB1, GIT1, GRK2, GSK3A, GTF2F1, GUK1, HGFR / MET, HGS, HIPK1, HSP90AB1, ICK, IGF-1 R, IKK-E / IKBKE, ILK, INPP5D, INPPL1, Insulin R / CD220, IRAK1, IRAK4, Itk, ITSN2, JAK2, JAK3, JUN, KSR1, KSR2, LATS1, LCK, LIMK1, LMTK2, LTK, Lyn, MAK, MAP2K1, MAP2K3, MAP2K4, MAP2K5, MAP2K7, MAP3K11, MAP3K12, MAP3K2, MAP3K20, MAP3K5, MAP3K7, MAP3K8, MAP4K2, MAP4K4, MAP4K5, MAPK1, MAPK11, MAPK13, MAPK3, MAPK4, MAPK6, MAPK7, MAPK8, MAPKAPK2, MAPKAPK3, MAPKAPK5, MARK1, MARK3, M-CSF R, MELK, Mer, MOS, MSK1, MSP Receptor / Ron, mTOR, MUSK, NEDD9, NEK2, NEK6, NEK7, NLK, NSE, NTRK1, NUAK1, OXSR1, PAK1, PAK2, PAK4, PAK5, PBK, PCYT1A, PCYT1B, PDGF R alpha, PDLIM5, PDPK1, PEAK1, PECAM-1 / CD31, PFN1, PGK1, PIK3R1, PIK3R2, PIKFYVE, PIM2, PKM, PKN1, PLCG1, PLCG2, PLK1, PLK4, PPP1R11, PRAG1, PRKAA1, PRKAA2, PRKACA, PRKCA, PRKCB, PRKCD, PRKCQ, PRKCZ, PRKD1, PRKD2, PRKG1, PRKX, PRMT5, PRPF4B, PTK2B, PTK6, RAF1, RAGE, Ret, RIOK1, RIPK1, RIPK2, RIPK4, ROCK1, ROCK2, ROR2, ROS1, RPS6, RPS6KA1, RPS6KA2, RPS6KA4, RPS6KB1, RPS6KB2, SCF R / CD117 / c-kit, SCYL1, SIK1, SIK2, SIK3, SIT1, SLK, SMG1, snRNP70, Src, SRPK1, STAM2, STK10, STK11, STK24, STK25, STK36, STK38, STK4, SYK, TAOK1, TBC1D7, TBK1, Tec, Tie-2 / TEK, TLN1, TNK1, TNK2, Transglutaminase 2 / TGM2, TRIM28, TRIM33, TRKB, TRKC, TSSK3, TTK, TWF1, TWF2, TXK, Tyk2, TYRO3, VAV1, VEGFR1, VEGFR2, VEGFR3, VIM, WAS, WEE1, WNK1, YES1, ZAP70
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
- RayBio® L-Series Antibody Array Glass Slide(s)
- Spin Columns
- Labeling Reagent
- Stop Solution
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Streptavidin-Conjugated Fluor
- Adhesive Plastic Strips
- Labeling Buffer
- Lysis Buffer
- 30 ml Centrifuge Tube
Other Materials Required
- Distilled or de-ionized water
- Small plastic or glass containers
- Orbital shaker or oscillating rocker
- 1 ml tube
- Pipettors, pipette tips and other common lab consumables
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Purify Samples with Spin Column
- Biotinylate Samples
- Purify biotinylated samples using spin column
- Dry the glass slide
- Block array surface
- Incubate with Samples
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAH-BLG-PHO1.gal)
Excel (AAH-BLG-PHO1_Analysis_Tool.xlsx)