Reagents and instruments for immunology, cell biology and molecular biology.
 
> Plant hormone abscisic acid,ABA ELISA Kit

Plant hormone abscisic acid,ABA ELISA Kit

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Cat# CSB-E09159Pl-96T

Size : 96T

Brand : Cusabio

Contact local distributor :


Phone : +1 850 650 7790

CSB-E09159Pl
Size 96T,5×96T,10×96T

Product Details

Abbreviation
ABA
Species
Plant
Sample Types
plant tissues
Detection Range
0.156 μg/ml-10 μg/ml.
Sensitivity
0.04 μg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<10%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<20%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Recovery
The recovery of plant ABA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Xylem saps from plants (n=5) 95 89-98  
Crude extracts (n=5) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
10 0.124 0.126 0.125    
5 0.213 0.228 0.221    
2.5 0.357 0.361 0.359    
1.25 0.498 0.506 0.502    
0.625 0.686 0.701 0.694    
0.312 1.244 1.268 1.256    
0.156 1.510 1.534 1.522    
0 2.241 2.297 2.269    
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Plant ABA ELISA Kit was designed for the quantitative measurement of Plant ABA protein in plant tissues. It is a Competitive ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.04 μg/mL.

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