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Reagents and instruments for immunology, cell biology and molecular biology.

Propidium Iodide [25535-16-4]

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Cat# NB-64-10859-5mg

Size : 5mg

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Phone : +1 850 650 7790

Brand : Neo Biotech

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Phone : +1 850 650 7790

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Propidium Iodide

(Synonyms: PI) Copy Product Info

Synonyms: PI

Catalog No. T2130 Copy Product Info

Purity: 99.46%

Propidium Iodide (PI) is a red fluorescent dye utilized for cell staining and is suitable for fluorescence microscopy, confocal microscopy, flow cytometry, and fluorometer analysis. In aqueous solution, the Ex/Em of PI is 493/636 nm. Upon binding with nucleic acid, the Ex/Em shifts to 535/617 nm, enhancing the fluorescence signal 20-30 times.

Cas No. 25535-16-4

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.46%

Color:Red to Purple

Product Introduction

Propidium Iodide AI Summary

Propidium Iodide exhibits a diverse range of bioactivities. It shows strong binding affinity for DNA at pH 7, with a measured binding constant greater than 5.0 M^-1. In relation to acetylcholinesterase (AChE), Propidium Iodide has a dissociation constant (Ki) of 1100.0 nM in mouse and inhibits human AChE with an IC50 value of 32.3 µM. Additionally, it demonstrates inhibitory activity against human butyrylcholinesterase (BChE) with an IC50 value of 13.2 µM. The compound also demonstrates an extensive capability to inhibit amyloid beta aggregation; it inhibits human recombinant AChE-induced amyloid beta (1-40) aggregation by 82% at 100 µM, and self-induced amyloid beta (1-40) aggregation by 61.1% at 10 µM. Furthermore, Propidium Iodide exhibits moderate binding affinity for the NMDA receptor and affects calcium signaling pathways. In the context of antiviral activity, the compound inhibits SARS-CoV-2 induced cytotoxicity in Caco-2 cells by 15.98% at a concentration of 10 µM, and shows significant inhibition (76.32%) of the SARS-CoV-2 3CL-Pro protease at 20 µM. Overall, Propidium Iodide demonstrates potential therapeutic implications in neurodegenerative diseases like Alzheimer's and as an antiviral agent against SARS-CoV-2..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Propidium Iodide (PI) is a red fluorescent dye utilized for cell staining and is suitable for fluorescence microscopy, confocal microscopy, flow cytometry, and fluorometer analysis. In aqueous solution, the Ex/Em of PI is 493/636 nm. Upon binding with nucleic acid, the Ex/Em shifts to 535/617 nm, enhancing the fluorescence signal 20-30 times.
Targets & IC50	HEK293 cells:1.1 μM
In vitro	METHODS: Propidium Iodide uptake and Flow cytometry to detect cell death: 1. Store the PI stock solution (0.5 mg/mL in PBS) in a dark place at 4℃. Immediately before use, prepare PI-FACS buffer by adding 20 µL of PI stock solution per 1 mL of PBS. 2. Collect suspended cells: Collect cells directly in centrifuge tubes and centrifuge at 500g for 5 min to harvest all cells. 3. Collect adherent cells: Remove and preserve the medium containing dead and mitotic cells. Isolate live cells using standard tissue culture techniques, such as incubation with trypsin-EDTA, and be sure to collect any washings (e.g., PBS). Add cells from the culture medium and cells from any wash solution to the isolated cells and centrifuge at 500 g for 5 min to harvest all cells. 4. Resuspend the harvested cells in PI-FACS buffer. The cells were incubated in the dark for 15 min at room temperature. 5. Determine the cell mortality rate by flow cytometry. [1] The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	PI
Cell Research	Propidium Iodide absorption and flow cytometry assay for cell death 1. Store PI stock solution (0.5 mg/mL in PBS) at 4°C in the dark. Immediately before use, prepare PI-FACS buffer by adding 20 µL of PI stock solution to every 1 mL of PBS. 2. Collect suspended cells: Collect cells directly in a centrifuge tube and harvest all cells by centrifugation at 500g for 5 min. 3. Collect adherent cells: Remove and save the culture medium containing dead and mitotic cells. Isolate live cells using standard tissue culture techniques, such as incubation with trypsin-EDTA, and be sure to collect any wash solution (such as PBS). Add cells in the culture medium and cells from any wash solution to the isolated cells and harvest all cells by centrifugation at 500g for 5 min. 4. Resuspend the harvested cells in PI-FACS buffer. Incubate the cells in the dark at room temperature for 15 min. 5. Determine cell death rate by flow cytometry. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

Chemical Properties

Molecular Weight	668.39
Formula	C₂₇H₃₄I₂N₄
Cas No.	25535-16-4
Smiles	[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c2cc(N)ccc2c2ccc(N)cc12
Relative Density.	1.5072 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 5 mg/mL (7.48 mM), Sonication and heating to 60℃ are recommended.

DMSO: 126.3 mg/mL (188.96 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (4.94 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.4961 mL	7.4807 mL	14.9613 mL	74.8066 mL
5 mM	0.2992 mL	1.4961 mL	2.9923 mL	14.9613 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.1496 mL	0.7481 mL	1.4961 mL	7.4807 mL
20 mM	0.0748 mL	0.3740 mL	0.7481 mL	3.7403 mL
50 mM	0.0299 mL	0.1496 mL	0.2992 mL	1.4961 mL
100 mM	0.0150 mL	0.0748 mL	0.1496 mL	0.7481 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

Propidium IodidePropidiumInhibitorinhibit

Related Tags: Propidium Iodide chemical structure | Propidium Iodide in vitro | Propidium Iodide formula | Propidium Iodide molecular weight