Rabbit Anti-DNMT3A Polyclonal Antibody

Cat# HPA026588-25ul

Size : 25ul

Brand : Atlas Antibodies

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Phone : +1 850 650 7790

HPA026588
DNA (cytosine-5-)-methyltransferase 3 alpha

Anti-DNMT3A Antibody

Polyclonal Antibody against HUMAN DNMT3A

0.1 mg/ml
info@biovalley.fr

Immunohistochemical staining of human lymph node shows strong nuclear positivity.

Product details

  • Target protein: DNA (cytosine-5-)-methyltransferase 3 alpha
  • Target gene: DNMT3A
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000020661 (91%)RAT - ENSRNOG00000026649 (91%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000009353
  • Concentration: 0.1 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:50 - 1:200
    • Retrieval method: HIER pH6

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC) 

Target information

  • Protein name: DNA (cytosine-5-)-methyltransferase 3 alpha
  • Gene name: DNMT3A
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (8)

  • Bai W, Xu J, Gu W, Wang D, Cui Y, Rong W, Du X, Li X, Xia C, Gan Q, He G, Guo H, Deng J, Wu Y, Yen RW, Yegnasubramanian S, Rothbart SB, Luo C, Wu L, Liu J, Baylin SB, Kong XNat Commun , 2025 Jan 8; 16:474. Epub 2025 Jan 82025 Jan 8

  • Xu S, Yuan Z, Jiang C, Chen W, Li Q, Chen TAdv Sci (Weinh) , 2024 Feb 21; 11(16):2308531. Epub 2024 Feb 212024 Feb 21