Endotoxin removal kits

The endotoxins are small hydrophobic molecules, lipopolysaccharides (LPS), associated with the outer membranes of certain microorganisms and more particularly the Gram-negative bacteria (Brucella, Neisseria, Vibrio ...). These small thermostable molecules are not secreted but are released only when the cells are destroyed. In large quantities, endotoxins can cause haemorrhagic shock and acute diarrhea, lower levels may cause fever, lower resistance to bacterial infections, leukopenia followed by leukocytosis and other biological effects, depending of organism and its physiological conditions.

Small amounts of endotoxins in recombinant protein or antibody production can cause tissue damage and even death of the host organism. Endotoxins should therefore be removed from drugs, injectables and other biological products in order to avoid these effects.

The effects of endotoxins vary from one organism or cell type to another. This is due to the different cellular responses to endotoxins. The critical level of endotoxins is therefore very variable according to the organisms or cell types, which is why it is preferable to have an endotoxin level as low as possible.

Spin columns	NoEndo M	NoEndo S	NoEndo HC
Typical in situ binding capacity per column	3,000 EU	30,000 EU	500,000-1,000,000 EU
Typical endotoxin binding capacity	300 EU/ml	1,500 EU/ml	30,000 EU/ml
Minimum endotoxin levels tested post-column	<0.03 EU/ml	<0.05 EU/ml	<0.05 EU/ml
Typical endotoxin clearance after 1 pass	-	3 log reduction	3 log reduction
Typical endotoxin clearance after 2 passes	-	4 log reduction	4 log reduction
Typical endotoxin clearance after 1 hour incubation	2 log reduction	-	-
Typical endotoxin clearance after 3 hour incubation	3 log reduction	-	-
Maximum sample load volume	20 ml	20 ml	20 ml
Bed volume	0.25 ml loose	1 ml pre-packed	1.7 ml pre-packed
Resin	NoEndo resin	NoEndo resin	NoEndo resin
Bead size range	100 μm	100 μm	100 μm
Proteus matrix	Cross-linked 6 % agarose	Cross-linked 6 % agarose	Cross-linked 6 % agarose
Recommended working pH	4-8	4-8	4-8
Colour coded end-caps	Supplied in vials	Light green	Dark green

Frequently Asked Questions (FAQ)

How can I quickly find a endotoxin product?

To find a endotoxin product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Biovalley stock several product codes in the endotoxin category?

Yes, Biovalley distributes several product references in the endotoxin category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for endotoxin products?

Yes, you can request a quote for endotoxin products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a endotoxin product is available to order?

The availability of a endotoxin product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.

What should I do if I cannot find the endotoxin product I am looking for?

If the endotoxin product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all endotoxin products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.