FPLC columns for GST tagged proteins
Glutathione S-transferase (GST) is a 211 amino acid protein (26kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result of expression from this vector is a GST-tagged fusion protein in which the functional GST protein (26kDa) is fused to the N-terminus of the recombinant protein.
Because GST rapidly folds into a stable and highly soluble protein upon translation, inclusion of the GST tag often promotes greater expression and solubility of recombinant proteins than expression without the tag. In addition, GST-tagged fusion proteins can be purified or detected based on the ability of GST (an enzyme) to bind its substrate, glutathione (GSH).
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.
Elution of the GST-tagged protein is performed with free glutathione in the elution buffer. The affinity of Glutathione-S-Transferase for free glutathione is higher than the one for the immobilized glutathione bound to the agarose matrix. Therefore free glutathione replaces the immobilized glutathione which leads to release of the GST-tagged protein from the matrix.
Frequently Asked Questions (FAQ)
Where can I find gst tagged proteins products on the Biovalley website?
gst tagged proteins products are available in this section and can be sorted by product families or sub-categories, such as GST FPLC Columns. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the gst tagged proteins category?
The gst tagged proteins category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The GST FPLC Columns sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a gst tagged proteins product?
To find a gst tagged proteins product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Biovalley stock several product codes in the gst tagged proteins category?
Yes, Biovalley distributes several product references in the gst tagged proteins category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for gst tagged proteins products?
Yes, you can request a quote for gst tagged proteins products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a gst tagged proteins product is available to order?
The availability of a gst tagged proteins product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.
What should I do if I cannot find the gst tagged proteins product I am looking for?
If the gst tagged proteins product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all gst tagged proteins products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.