Reagents and instruments for immunology, cell biology and molecular biology.
 
Histone modification analysis

Histone modification analysis

Histone modification analysis examines post-translational changes on histone proteins that regulate chromatin structure and gene expression.

These dynamic marks—primarily methylation, acetylation, phosphorylation, ubiquitination, and sumoylation—occur on specific lysine, arginine, and serine residues, creating combinatorial "codes" interpreted by chromatin readers.

Core Methodologies

Chromatin Immunoprecipitation (ChIP) selectively isolates histone-DNA complexes:

  • Fixation preserves protein-DNA interactions
  • Chromatin shearing generates 150-300 bp fragments
  • Antibody pulldown enriches modified histone fractions
  • Quantitative PCR or sequencing reveals genomic occupancy

Western Blotting provides semi-quantitative modification levels from acid-extracted histones.

ELISA Assays deliver high-throughput, absolute quantification of specific marks from nuclear extracts.

Mass Spectrometry profiles combinatorial modifications with single-residue resolution.

Major Histone Modifications

Actylation (H3K9/14/18/27, H4K5/8/12/16):

  • Neutralizes lysine charge, opens chromatin
  • Recruited by HATs (p300/CBP), removed by HDACs/SRTs
  • Associates with active promoters and enhancers

Methylation (H3K4me1/2/3, H3K9me1/2/3, H3K27me1/2/3, H4K20me1/2/3):

  • H3K4me3 marks active promoters
  • H3K27me3 silences developmental genes (Polycomb)
  • H3K9me3 mediates heterochromatin formation
  • Writers: SET-domain HMTs; erasers: KDMs/JmjC

Phosphorylation (H3S10, H3T11):

  • Mitosis/chromatin condensation marker
  • Immediate-early gene activation

Frequently Asked Questions (FAQ)

Where can I find histone modification analysis products on the Biovalley website?

histone modification analysis products are available in this section and can be sorted by product families or sub-categories, such as Histone acetylation assays, Histone extraction kits, Histone methylation assays. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.

What types of products are available in the histone modification analysis category?

The histone modification analysis category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Histone acetylation assays, Histone extraction kits, Histone methylation assays sub-categories allow you to find the products that meet your needs more quickly.

How can I quickly find a histone modification analysis product?

To find a histone modification analysis product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Biovalley stock several product codes in the histone modification analysis category?

Yes, Biovalley distributes several product references in the histone modification analysis category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for histone modification analysis products?

Yes, you can request a quote for histone modification analysis products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a histone modification analysis product is available to order?

The availability of a histone modification analysis product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.

What should I do if I cannot find the histone modification analysis product I am looking for?

If the histone modification analysis product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all histone modification analysis products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.