Kits for the preparation of DNA libraries for NGS
Library preparation for Illumina is composed of 4 main steps, the progress of which can vary according to the methods used:
1. DNA fragmentation
DNA is fragmented either enzymatically or by sonication to create smaller strands
2. End repair
Because DNA fragmentation does not result in homogeneous, blunt-ended fragments, end repair is needed to ensure that each molecule is free of overhangs, and contains 5' phosphate and 3' hydroxyl groups
3. Adapter ligation
Adaptors (short, double-stranded pieces of synthetic DNA) are then ligated to these fragments with the help of DNA ligase. The adaptors enable the sequence to become bound to a complementary counterpart. These adapters contain sequence motifs that are required for subsequent steps (clonal amplification and the actual sequencing).
4. Library amplification
Library amplification is required so that the received signal from the sequencer is strong enough to be detected accurately. The Illumina technology uses the "Bridge PCR" method.
Frequently Asked Questions (FAQ)
Where can I find kits products on the Biovalley website?
kits products are available in this section and can be sorted by product families or sub-categories, such as Difficult samples, Bi-Seq, Genome. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the kits category?
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To find a kits product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Biovalley stock several product codes in the kits category?
Yes, Biovalley distributes several product references in the kits category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for kits products?
Yes, you can request a quote for kits products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a kits product is available to order?
The availability of a kits product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.
What should I do if I cannot find the kits product I am looking for?
If the kits product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all kits products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.