Kits for the preparation of Illumina libraries - Difficult samples

Kits for the preparation of Illumina libraries - Difficult samples


The first step of a Next Generation Sequencing (NGS) is the preparation of a DNA bank. And for this NGS sequencing to be successful, the DNA bank must be of high quality. However, not all samples will yield good quality DNA for a good DNA bank. We then talk about difficult samples. These include FFPE samples, cell-free DNA samples or very small samples (also called single cells). Although these samples deliver DNA of poor quality and/or in very small quantities, their analysis gives results that are not possible with other samples. Examples include circulating tumour DNA or tumour biopsies. When starting with DNA in limited quantities or of variable quality, the bank preparation method must be precise and efficient, avoiding purification steps that reduce sample recovery. 

Frequently Asked Questions (FAQ)

Where can I find difficult samples products on the Biovalley website?

difficult samples products are available in this section and can be sorted by product families or sub-categories, such as Cell free DNA (cfDNA), FFPE samples. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.

What types of products are available in the difficult samples category?

The difficult samples category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The FFPE samples, Cell free DNA (cfDNA) sub-categories allow you to find the products that meet your needs more quickly.

How can I quickly find a difficult samples product?

To find a difficult samples product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Biovalley stock several product codes in the difficult samples category?

Yes, Biovalley distributes several product references in the difficult samples category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for difficult samples products?

Yes, you can request a quote for difficult samples products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a difficult samples product is available to order?

The availability of a difficult samples product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.

What should I do if I cannot find the difficult samples product I am looking for?

If the difficult samples product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all difficult samples products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.