Kits for the preparation of Illumina libraries - ATAC-Seq

ATAC-seq stands for Assay for Transposase-Accessible Chromatin with highthroughput sequencing. It is a technique used in molecular biology for mapping chromatin accessibility sites. The ATAC-Seq method is based on the construction of a new generation sequencing library (NGS) using hyperactive Tn5 transposase. The principle of ATAC-seq is based on the mode of action of the Tn5 transposase on the genomic DNA of the sample. Transposases are enzymes that catalyse the movement of transposons to other parts of the genome. Although natural transposases have a low level of activity, ATAC-seq uses a hyperactive mutated transposase.

The principle of the method consists of incubating the chromatin in the presence of Tn5 transposomes, to simultaneously fragment and index the exposed DNA fragments. This simple and rapid protocol must however be carried out delicately so as not to modify the structure of the chromatin and reveal sites to the transposomes.

The main advantages of ATAC-Seq over other techniques, such as FAIRE-Seq or DNase-Seq, which study similar chromatin characteristics, are the smaller number of cells required for the test and the relative simplicity of its two-step protocol.

Frequently Asked Questions (FAQ)

How can I quickly find a atac-seq product?

To find a atac-seq product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Biovalley stock several product codes in the atac-seq category?

Yes, Biovalley distributes several product references in the atac-seq category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for atac-seq products?

Yes, you can request a quote for atac-seq products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a atac-seq product is available to order?

The availability of a atac-seq product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.

What should I do if I cannot find the atac-seq product I am looking for?

If the atac-seq product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all atac-seq products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.