Kits for the preparation of Illumina libraries - Bisulfite sequencing (Bi-Seq)

Bisulphite sequencing (BiSeq) involves the use of sodium bisulphite DNA treatment prior to sequencing. The purpose of this treatment is to determine the DNA methylation profile. Sodium bisulphite deaminates unmethylated cytosines faster than methylated cytosines. This treatment, which converts the cytosines into uracils and then into thymines through amplification, makes it possible to transform the epigenetic mark into a genetic variation that can be identified by sequencing. New generation sequencing (NGS) now makes it possible to study the methylome (methylated DNA) on the entire genome with high resolution (of the order of a base pair). DNA methylation is one of the most studied epigenetic modifications. Indeed, this process plays an important role in the regulation of gene expression and therefore in many biological processes and in a wide range of pathologies.

The bisulphite sequencing protocol consists of preparing DNA fragments by sonication, indexing them with methyl adapters and then selecting them by size. The libraries are then treated with sodium bisulphite, amplified and sequenced. The conversion reaction can be checked by integrating unmethylated control DNA.

Frequently Asked Questions (FAQ)

How can I quickly find a bi-seq product?

To find a bi-seq product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Biovalley stock several product codes in the bi-seq category?

Yes, Biovalley distributes several product references in the bi-seq category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for bi-seq products?

Yes, you can request a quote for bi-seq products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a bi-seq product is available to order?

The availability of a bi-seq product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.

What should I do if I cannot find the bi-seq product I am looking for?

If the bi-seq product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all bi-seq products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.