Preparation of DNA libraries for Ion Torrent NGS
Next Generation Sequencing (NGS) is a powerful tool for large scale, high definition sequencing. There are different types of sequencing. The most widely used technology at present is the synthetic sequencing technology (or Illumina sequencing). But there are also other technologies such as Ion Torrent technology.
Ion Torrent sequencing is a sequencing method based on the detection of hydrogen ions released during DNA polymerisation. It is a synthetic sequencing method in which a complementary strand is created from a template. Ion Torrent sequencing consists of 4 main steps: building the bank, amplification, sequencing and analysis. Unlike Illumina sequencing, Ion Torrent or Ion Proton sequencing does not use optical signals. Rather, it exploits the fact that the addition of a dNTP to a DNA polymer releases an H+ ion. As in other types of NGS technologies, the input DNA or RNA is fragmented into fragments of about 200bp. Adapters are added and a molecule is placed on a bead. The molecules are amplified on the bead by emulsion PCR. Each bead is then placed in a single well of a slide.
Frequently Asked Questions (FAQ)
Where can I find ion torrent products on the Biovalley website?
ion torrent products are available in this section and can be sorted by product families or sub-categories, such as Kits. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the ion torrent category?
The ion torrent category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Kits sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a ion torrent product?
To find a ion torrent product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Biovalley stock several product codes in the ion torrent category?
Yes, Biovalley distributes several product references in the ion torrent category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for ion torrent products?
Yes, you can request a quote for ion torrent products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a ion torrent product is available to order?
The availability of a ion torrent product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.
What should I do if I cannot find the ion torrent product I am looking for?
If the ion torrent product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all ion torrent products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.