Quantitative RT-PCR - qRT-PCR
RT-qPCR is a technique which makes it possible to make a quantitative PCR (polymerase chain reaction) from an RNA sample. RNA is first retrotranscribed by an enzyme called reverse transcriptase, which allows the synthesis of the complementary DNA (cDNA). The latter is then used to carry out a quantitative PCR. Reverse transcriptase or reverse transcriptase (RT) is an enzyme used by retroviruses and retrotransposons that transcribe the genetic information of viruses or retrotransposons from RNA to DNA, which can integrate into the genome of the host.
RT-qPCR has been developed to use RNAs as a PCR amplification matrix. It is certainly the most sensitive method for detecting (and possibly quantifying) messenger RNAs at the level of an organ, tissue or cell. It is also used for the construction of cDNA libraries, sorting of mRNA (Differencial Display RT-PCR) as well as the construction of DNA probes.
Quantitative PCR or real-time qPCR is a particular method of polymerase chain reaction to measure the initial amount of DNA. In fact, quantitative PCR measures the number of amplicons (DNA portion defined by a pair of primers).
It enables continuous monitoring ("in real time") of the PCR amplification process by detecting the fluorescence emitted by the neo-formed PCR products.
The profile of a conventional quantitative PCR reaction can be broken down into 3 steps:
- A first step known as background noise: the amount of amplified fragment is insufficient to generate a fluorescent signal greater than the background noise (and therefore the fluorescence generated).
- A second step of exponential phase of growth: the quantity of amplified fragment generates a fluorescent signal higher than the detection threshold of the apparatus, and then the number of amplified products doubles on each cycle. In logarithmic coordinates, this phase is represented by a line.
- A final stage of the plateau phase: certain components of the reaction (and in particular the number of available Taq molecules) become limiting. The system no longer allows exponential amplification.
The detection can be carried out using SYBR green, a fluorescent organic compound which binds to nucleic acids, or probes, Taqman or FRET.
Frequently Asked Questions (FAQ)
Where can I find qrt-pcr products on the Biovalley website?
qrt-pcr products are available in this section and can be sorted by product families or sub-categories, such as Mix for probes for RT-qPCR, RT-qPCR SYBR Green Mixes. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the qrt-pcr category?
The qrt-pcr category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Mix for probes for RT-qPCR, RT-qPCR SYBR Green Mixes sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a qrt-pcr product?
To find a qrt-pcr product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Biovalley stock several product codes in the qrt-pcr category?
Yes, Biovalley distributes several product references in the qrt-pcr category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for qrt-pcr products?
Yes, you can request a quote for qrt-pcr products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a qrt-pcr product is available to order?
The availability of a qrt-pcr product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.
What should I do if I cannot find the qrt-pcr product I am looking for?
If the qrt-pcr product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all qrt-pcr products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.