Galanthus nivalis (Snowdrop Bulb) (GNA)

Cat# G1044-01-5mg

Size : 5mg

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Grade
Molecular Biology Grade

Applications
E

Shipping Temp
Blue Ice

Storage Temp
-20°C

Galanthus nivalis lectin is a small molecular weight tetramer consisting of subunits of about 13,000D. It has an isoelectric point below pH 4.6. The lectin contains little or no carbohydrate and, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++ or Mn++ for binding. Binding seems to be preferentially directed toward structures containing (a-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind alpha linked glucose. Reports indicate that this lectin binds IgM but not IgG of the immunoglobulin classes of rat and mouse. The only protein from human serum reported to bind to this lectin is a2-macroglobulin.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Inhibiting/Eluting Sugar:
100mM-200mM a-methyl mannoside

Storage and Stability:
Lyophilized powder may be stored after receipt at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Purity
Purified by SDS-PAGE

Concentration
Not determined

Form
Supplied as a lyophilized powder, with ~15% salts. We recommend using 10mM HEPES buffered saline, pH 8.5, 0.1mM Ca++ for reconstitution and diluting. For preserving solutions at 4ºC, 0.04% sodium azide should be used.


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.