Lens culinaris LCA (FITC)
Size : 1mg
Brand : US Biological
GradeMolecular Biology Grade
Shipping TempBlue Ice
Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.
Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.
50-200ug/ml will agglutinate type O human erythrocytes. 2-5ug/ml will agglutinate neuraminidase treated cells.
D-Mannose and D-Glucose. A fucose alpha (1,6) to the core GlcNAc of N-linked glycopeptides is an important determinant for lectin activity.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Supplied as a liquid in 0.05M Tris, 0.15M sodium chloride, pH 7.1, 0.05% sodium azide. Labeled with FITC.
Protein Concentration (OD 280)
Gel filtration performed after conjugation to remove free FITC.