Kits and reagents for mycoplasma detection and elimination
Mycoplasma, bacteria, viruses - the typical contaminants of cell cultures can have unidentified substantial effects on the reproducibility of research results. Protect your cells from contaminants with culture supplements and routine testing with appropriate test kits to be confident in your research results.
Détection
Minerva Biolabs offers high-quality but reasonable diagnostic kits for the detection of microbial contaminants in cell cultures, biopharmaceuticals, clinical samples. These kits use the sensitivity of conventional PCR techniques and real-time control to ensure superior contamination.
Mycoplasma Detection Kits for conventional and qPCR
Venor®GeM Classic allows fast, reliable and time-saving routine monitoring of mycoplasma contamination by PCR.
Venor®GeM Advance contains PCR reaction tubes pre-coated with all PCR reagents to reduce the total assay time without need to prepare aliquots of a master mix. For additional convenience the gel loading buffer and dye are already included in the reaction buffer. After thermal cycling the PCR can be loaded directly on the agarose gel.
Venor®GeM qEP utilizes quantitative, real-time PCR (qPCR). The kit can be performed with any type of real-time PCR cycler able to detect the fluorescence dyes FAM and HEX. The protocol provided is preferred for fast and reliable screening of cell culture supernatants most applicable in research and development. The detection procedure can be performed within 3 hours.
Venor®GeM OneStep Venor®GeM OneStep is a complete kit which includes all reagents required for PCR. Primer, nucleotides, polymerase and the internal amplification control are provided ready-to-use in a lyophilized reaction mix. The included rehydration buffer is added to the mix, aliquots made according to the sample number, sample or Positive Control DNA added and the setup is ready for PCR.
Onar® Bacteria PCR kit for conventional, endpoint PCR of bacteria in various in situ biologicals including cell cultures and virus stocks.
Eliminition
Mynox ® is the first biological reagent that actually eliminates mycoplasma by killing them. It is effective with only one treatment. The activity of Mynox ® is based on its biophysical properties, making the development of resistant strains highly unlikely and are easy disposal after treatment.
Classic Mynox® is an antibiotic-free formulation; its activity is based on a biophysical mechanism, thus there is no development of resistant strains. Mycoplasma are permanently destroyed within 2-3 hours.
Mynox®Gold represents the further development of the classical Mynox®. Mynox®Gold is a combination of a standard antibiotic and the biological Mynox® reagent. One application comprises of a starter treatment that eliminates most of the mycoplasma particles without harming the cells. The main treatment kills all remaining mycoplasma.
Mycoplasma-Off is used for cleansing and disinfection of all laboratory surfaces and apparatus, including clean benches, incubators, work benches, cell storage boxes and liquid nitrogen containers from mycoplasma contamination.
Mycoplasma-Off Wipes are the simple solution for cleaning and disinfecting a variety of laboratory surfaces at the same time. There's no need for multiple products when you have the convenience of a simple-to-use wipe. When you use a sponge or even paper towel, you can spread bacteria from one surface to another. Instead of killing germs, you are pushing them around and potentially increasing the contaminated surface area.
Prevention
ZellShield® protects cell cultures from a broad range of common contaminants. The extremely powerful combination of innovative antibiotics is blocking the bacterial DNA synthesis, the protein biosynthesis or shows macrolidic properties. These bactericidal features allow the permanent removal of the contaminants from the culture. The use of old fashioned antibiotics like penicillin/streptomycin, nystatin or amphotericin B is no longer required!